cell library

鍗氶泤杈戝洜杩愮敤楂樻晥鐨凜RISPR鍩哄洜缂栬緫鎶鏈紝鍚屾椂缁撳悎楂橀氶噺閬椾紶绛涢夌殑鍘熺悊锛屽湪鎴愬姛鏋勫缓鍑簊gRNA鏂囧簱鍚庯紝瀵圭粏鑳炵郴杩涜绛涢夊伐浣滐紝鑾峰緱绛涢夊悗鐨勭粏鑳炴枃搴撱傜粏鑳炴枃搴撶殑鎺ㄥ嚭杩涗竴姝ョ畝鍖栦簡绉戠爺浜哄憳鐨勫伐浣滐紝搴旂敤缁嗚優鏂囧簱鍙洿鎺ヨ繘琛岄珮鍐呮兜绛涢変互鍙奛GS绛涢夛紝璁╃鐮旂殑杩涘害鏇村揩涓绋嬨

鍗氶泤杈戝洜浣跨敤鍙岃川绮掔郴缁熼鍏堟瀯寤篐T-29鐨凜as9绋冲畾琛ㄨ揪缁嗚優绯伙紝鐒跺悗鍐嶄娇鐢ㄤ汉绫诲叏鍩哄洜缁勬枃搴撹繘琛屾參鐥呮瘨渚垫煋锛岄氳繃鎶楁х瓫閫夎幏寰楀崟涓粏鑳炲崟涓熀鍥犳暡闄ょ殑鍏ㄥ熀鍥犵粍鏁查櫎缁嗚優鏂囧簱銆傜粏鑳炴枃搴撻氳繃NGS妫娴嬶紝纭繚缁嗚優鏂囧簱鐨勮鐩栧害瀹屾暣搴︼紝缁嗚優鏂囧簱瑕嗙洊搴︿笉浣庝簬500鍊嶃

浜у搧缁勬垚

缁嗚優鍚嶇О锛欻T-29 浜虹粨鑲犵檶缁嗚優銆丷D 浜烘伓鎬ц儦鑳庢í绾硅倢鐦ょ粏鑳炪並yse150 椋熺鐧岀殑槌炵姸缁嗚優鐧屻乀E-1 椋熺鐧屻丠CC1937 浜轰钩鑵虹檶缁嗚優绛
缁嗚優鏉ユ簮锛氫汉婧
闈跺熀鍥犳暟鐩細鏍规嵁缁嗚優鏂囧簱绫诲瀷鐨勪笉鍚岋紝闈跺熀鍥犳暟閲忎笉鍚
sgRNA 鏁扮洰锛氶拡瀵规瘡涓熀鍥犺璁12涓猻gRNA銆
瑙勬牸锛2X107 cells/tube
鐢熼暱鐗规э細璐村
鍩瑰吇鍩猴細鏍规嵁缁嗚優绫诲瀷纭畾涓嶅悓鍩瑰吇鍩烘潯浠
鍩瑰吇鏉′欢锛37鈩冿紝5% CO2锛孭H 鍊 7.2~7.4锛屾棤鑿屾亽娓╁煿鍏

璐ㄧ矑閮ㄥ垎

1. Cas9绋冲畾杞煋缁嗚優绯荤殑璐ㄧ矑

Cas9绋冲畾杞煋缁嗚優绯荤殑璐ㄧ矑

2. Edi-CELLTM 缁嗚優鏂囧簱鏋勫缓璐ㄧ矑

3銆 Edi-CELLTM 缁嗚優鏂囧簱鏋勫缓浣跨敤鐨勭梾姣掑寘瑁呰川绮抪Helper 1鍜宲Helper 2

pHelper 1鍜宲Helper 2

缁嗚優鏂囧簱浜у搧鍒楄〃

璐у彿 浜у搧鍚嶇О 闈跺熀鍥犳暟閲 缁嗚優绯 澶囨敞
HTSCL2202201 Edi-CELLTM HT-29 Whole Genome CRISPR Knock-out Cell Library 18823 HT-29 璇存槑涔
HTSCL2202202 Edi-CELLTM RD Whole Genome CRISPR Knock-out Cell Library 18823 RD 璇存槑涔
HTSCL2202206 Edi-CELLTM HCC1937 Kinase & Phosphotase CRISPR
Knock-out Cell Library
1003 HCC1937 璇存槑涔
HTSCL2202207 Edi-CELLTM HCC1937 Druggable CRISPR
Knock-out Cell Library
2897 HCC1937 璇存槑涔

缁嗚優鏂囧簱瀹氬埗

鍙寜鐓у疄楠岄渶姹傚畾鍒剁浉鍏崇粏鑳炴枃搴擄紝璇︾粏璇㈤棶010-8073 3899-802锛屾垨鍙戦侀偖浠跺埌bd@edigene.com锛岃杩版偍鐨勫疄楠岄渶姹傘