Knockout thousands of genes at once

The simplicity of the CRISPR/Cas9 system of genome engineering has opened up the possibility of performing genome-wide targeted mutagenesis in cell lines, enabling screening for cellular phenotypes resulting from genetic aberrations1.The ability of CRISPR to generate genetic loss of function mutations not only increases the magnitude of any effect over currently employed RNAi techniques, but allows analysis over longer periods of time which can be critical for certain phenotypes1.

Integrating the Loss of Function High-throughput Genetic Screening and CRISPR/Cas9 technology, EdiGene can demonstrate unknown gene-drug connection, discover new disease related drug target, and analyze gene function. EdiGene has the rich experience in high throughput genome editing and own a series of libraries and sub-libraries, and can generate customized libraries to meet special need for high throughput screens to accelerate drug discovery and clinical trials.

Workflow

high-throught

 

1 Andrew R. Bassett, Lesheng Kong, Ji-Long Liu, A Genome-Wide CRISPR Library for High-Throughput Genetic Screening in Drosophila Cells, Journal of Genetics and Genomics, Volume 42, Issue 6, 20 June 2015, Pages 301-309, ISSN 1673-8527.

2 Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, etc. Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715.