Join the Revolution! Similar to Karry Mullis’ development of PCR, CRISPR/Cas9 genome editing is revolutionizing biomedical research. By more efficiently enabling insight into basic biology through gene function studies, delineating biological pathways, discovering new drugs, CRISPR/Cas9 is accelerating the translation of precision and personalized medicine from the bench to the bedside. At EdiGene, we focus on developing and providing CRISPR/Cas9 technology-based genome engineering products and services. Through our proprietary genome editing technology, we are producing and validating the largest collection of CRISPR/Cas9 ready-made knockout cell lines (Edi-G KO Cell Lines) and their derivative products such as whole cell lysates, cell pellets, total RNAs and more. We currently have over 1500 validated knockout cell lines generated in either HeLa or HEK293T cells. In addition to expanding the knockout gene list, we are expanding the knockout cell line collection to other common human cancer cell types including HCT116, HepG2, A549, and Jurkat cells and many more.
While the advent of CRISPR/Cas9 makes the genome editing process more simple and efficient, it can still take time to develop the material needed for successful genome editing. In order to expedite your genome editing process, we also provide validated reagents and tools including validated sgRNAs, whole genome and focused sgRNA libraries, and stably expressing Cas9 cells, etc. To learn more about how we can support your research, please click on any of the links below.
Undertaking genetic loss of function assays allows you to better understand how your phenotype of interest is impacted and can lead to greater systems biology insights. Using CRISPR/Cas9 genome editing technologies to knockout your gene or genes of interest is a powerful tool when studying gene function.
EdiGene’s ready-made knockout cell line collection contains over 1,600 (and rapidly growing!) validated cell lines and allows you to greatly simplify your gene editing experiments. The Edi-G KO Cell Lines are validated by PCR analysis and Sanger sequencing and are ready for immediate use.
You can further simplify and accelerate your antibody validation process using EdiGene’s KO Cell Lysates. In Western Blotting, a knockout lysate control serves to confirm whether a primary antibody recognizes its target antigen and determine whether the antibody binds to its target specifically.
The CRISPR/Cas9 system, consisting of a guide RNA (gRNA) and a Cas9 nuclease, allows researchers to create RNA-guided, site-specific DNA cleavage. EdiGene offers highly efficient CRISPR tools that can provide you with flexibility while simplifying your gene editing process.
Single guide or synthetic guide RNA (sgRNA) directs the Cas9 nuclease to create a DNA double strand break (DSB) at specific genomic location. The design of an effective, functional guide RNA is critical to achieving gene-specific knockout. The validated sgRNAs available from EdiGene are the same sgRNAs that have been proven to be effective in generated our industry leading KO Cell Lines. EdiGene’s validated sgRNA provide you with the ability to create knockouts across multiple cell types.
EdiGene's CRISPR/Cas9 Stable Cell Lines constitutively express Cas9 nuclease and provide a stable and readily available gene editing platform. Simply transfect or transduce your sgRNAs into the cells of your choice to produce the desired gene editing. The Cas9 Stable Cell Lines are available as HeLa and HEK293T cells.
EdiGene has developed proprietary CRISPR sgRNA and paired-guide RNA (pgRNA) knockout libraries that allow you to perform high-throughput loss of function screening assays in ready-made or customized sgRNA pools. Loss-of-function screening by gene knockout is a powerful systems biology tool that facilitates gene discovery, genome-scale functional interrogation (e.g. signal transduction pathways) and drug discovery (e.g. target identification and drug mechanism of action studies).
Pooled CRISPR sgRNA libraries are ideal for high-throughput screening when trying to validate known and uncover new drug targets. These libraries leverage the efficiency and specificity of the CRISPR gene editing technology to either knockout gene expression or transcriptionally activate genes.
· sgRNA Libraries
EdiGene’s Whole Genome sgRNA Library targets > 18,000 genes. Each gene has 8-10 sgRNAs. In addition to the whole genome sgRNA library, EdiGene offers sub-genome target libraries based on biological function and cellular localization.
· LncRNA Libraries
Long non-coding RNAs (lncRNAs) have been linked to cancer phenotypes, but their roles and mechanisms of action still remain unclear. Based on our proprietary paired-guide RNA (pgRNA) library design strategy, EdiGene has constructed an lncRNA-specific sgRNA library targeting ~ 700 lncRNAs4. The lncRNA library can help you discern the functional role of lncRNA in development, cancer, and other diseases.