Gene Symbol
AADAT
NCBI Gene ID
51166
Application
WB; Antibodies KO Validation
Other Names
KAT2
KATII
KYAT2
Gene Description
This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Quality control
Sanger Sequencing
Storage
Store at -20鈩 upon receiving. Lysate can be diluted with 1脳 SDS sample buffer and will be stable at -20鈩 for 12 months. Minimize freeze-thaw cycles.
Parental Cell Line
HEK293T
Reconstitution
To use as WB negative control, spin down briefly and resuspend in 100渭L 1脳 SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Components
1 vial of knockout cell lysate;
1 vial of parental cell lysate