Gene Symbol
ABCC3
NCBI Gene ID
8714
Application
WB; Antibodies KO Validation
Other Names
MLP2
MRP3
ABC31
MOAT-D
cMOAT2
EST90757
Gene Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Quality control
Sanger Sequencing
Storage
Store at -20鈩 upon receiving. Lysate can be diluted with 1脳 SDS sample buffer and will be stable at -20鈩 for 12 months. Minimize freeze-thaw cycles.
Parental Cell Line
HeLa
Reconstitution
To use as WB negative control, spin down briefly and resuspend in 100渭L 1脳 SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Components
1 vial of knockout cell lysate;
1 vial of parental cell lysate