Gene Symbol
ABL1
NCBI Gene ID
25
Application
WB; Antibodies KO Validation
Other Names
ABL
JTK7
p150
c-ABL
v-abl
c-ABL1
bcr/abl
Gene Description
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]
Quality control
Sanger Sequencing
Storage
Store at -20鈩 upon receiving. Lysate can be diluted with 1脳 SDS sample buffer and will be stable at -20鈩 for 12 months. Minimize freeze-thaw cycles.
Parental Cell Line
HeLa
Reconstitution
To use as WB negative control, spin down briefly and resuspend in 100渭L 1脳 SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Components
1 vial of knockout cell lysate;
1 vial of parental cell lysate