Gene Symbol
ACADVL
NCBI Gene ID
37
Application
WB; Antibodies KO Validation
Other Names
ACAD6
LCACD
VLCAD
Gene Description
The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Quality control
Sanger Sequencing
Storage
Store at -20鈩 upon receiving. Lysate can be diluted with 1脳 SDS sample buffer and will be stable at -20鈩 for 12 months. Minimize freeze-thaw cycles.
Parental Cell Line
HEK293T
Reconstitution
To use as WB negative control, spin down briefly and resuspend in 100渭L 1脳 SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Components
1 vial of knockout cell lysate;
1 vial of parental cell lysate