Gene Symbol
ANXA1
NCBI Gene ID
301
Application
WB; Antibodies KO Validation
Other Names
ANX1
LPC1
Gene Description
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
Quality control
Sanger Sequencing
Storage
Store at -20鈩 upon receiving. Lysate can be diluted with 1脳 SDS sample buffer and will be stable at -20鈩 for 12 months. Minimize freeze-thaw cycles.
Parental Cell Line
HeLa
Reconstitution
To use as WB negative control, spin down briefly and resuspend in 100渭L 1脳 SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Components
1 vial of knockout cell lysate;
1 vial of parental cell lysate