Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
NEFM Knockout 293T Cell Line 
NCBI Gene ID
WB; IHC; IF; Functional Assay
>95% viability before freezing. All cells were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins.
1 vial of knockout cell line
Parental Cell Line
Store at -80 centigrade upon receiving. Liquid nitrogen for a long-term storage. Minimizing freeze- thaw cycles.
1 bp insertion in exon 2
Insertion of the selection cassette in exon 2