Genomic Engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications.
CRISPR and Cas protein system is the latest tool in genome editing. CRISPR/Cas9 genome editing has become widely used due to its simplicity and versatility. It consist of a guide RNA (gRNA) and a Cas9 nuclease, allows researchers to create a RNA-guided site-specific DNA cleavage.
Creating stable cell lines is time-consuming and complex. Our research and development team have nearly a decade of experience working with a wide variety of cell backgrounds and executing nearly 2,000 gene editing experiments, access to a multiple genome editing technologies (CRISPR/Cas9, TALENs), EdiGene’s scientists will work with you to design your stable cell line and perform quality control testing to help ensure that the cell line meets your requirements. We optimize the cell culture conditions and transfection method, and we validate per your specifications, to help ensure you receive the edited cell line you desire.
EdiGene offers complete gene editing services from designing and cloning your gRNA and HR donor plasmid to generating a genome editing stable cell line. EdiGene is then able to use the double strand break created to modify your target gene by inducing knock-out, point mutation, or insertion. And our services were developed with you as our top priority. We provide a dedicated project manager who will work with you to help meet your research goals and timelines—we’re committed to helping you succeed.
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The simplicity of the CRISPR/Cas9 system of genome engineering has opened up the possibility of performing genome-wide targeted mutagenesis in cell lines, enabling screening for cellular phenotypes resulting from genetic aberrations1.The ability of CRISPR to generate genetic loss of function mutations not only increases the magnitude of any effect over currently employed RNAi techniques, but allows analysis over longer periods of time which can be critical for certain phenotypes1.
Integrating the Loss of Function High-throughput Genetic Screening and CRISPR/Cas9 technology, EdiGene can demonstrate unknown gene-drug connection, discover new disease related drug target, and analyze gene function. EdiGene has the rich experience in high throughput genome editing and own a series of libraries and sub-libraries, and can generate customized libraries to meet special need for high throughput screens to accelerate drug discovery and clinical trials.