CRISPR/Cas9 mediated genome editing is a powerful technique that allows you to create knock-in, knockout, and mutation of any gene, in any cell, in a highly-targeted manner and without introducing foreign DNA. It is only need a single guide RNA (sgRNA). Compared to previous methods of genome editing, such as TALENs and ZFN, CRISPR is much simpler and more efficiency.
single guide RNA (sgRNA) program Cas9 nucleases to cut at a specific genomic location. The design of an effective, functional guide RNA is critical to achieving specific gene knockout. Experimentally validated sgRNAs available from EdiGene are the guides that have been used to generate our Gene Knockout Cell Lines in HeLa or 293T cells successfully. If your cell type contains the target sequence, you can use these sgRNA directly to confidential your experiment.
sgRNA expression clones and lentiviral particles are available for targeting virtually any gene in any experimental system. sgRNA clones express either sgRNA only. Our validated sgRNA are all functionally validated in HeLa or 293T cell.
EdiGene Validated sgRNA enhance your experiment effeciency!
Search for the gene name in the search bar to find the validated sgRNA you interested.
Alternatively, if you can't find your cell line of interest, Contact Us.