Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

L0046099004
CL0046099004A
Antibody from OriGene.
SKU: TA811212
L0046099004-ABCF2-TA811105-02.jpg
CL0046099004A
Antibody from OriGene.
SKU: TA811105
CL0039896418A
Antibody from OriGene.
SKU: TA501224
CL0039896418A
Antibody from OriGene.
SKU: TA501221
CL0039896418A
Antibody from OriGene.
SKU: TA501238
CL0039896418A
Antibody from OriGene.
SKU: TA501222
CL0039896418A
Antibody from OriGene.
SKU: TA501216
CL0039896418A
Antibody from OriGene.
SKU: TA501223
CL0039896418A
Antibody from OriGene.
SKU: TA501220
CL0035743202A
Antibody from OriGene.
SKU: TA500072
CL0015573702A
Antibody from GeneTex.
SKU: GTX101358
CL0035009011A
Antibody from OriGene.
SKU: TA809490
CL0035009011A
Antibody from OriGene.
SKU: TA809455
CL0035009011A
Antibody from OriGene.
SKU: TA809478
CL0035009011A
Antibody from OriGene.
SKU: TA809482
CL0019478704A
Antibody from GeneTex.
SKU: GTX102312
CL0019478704A
Antibody from OriGene.
SKU: TA808924
CL0019478704A
Antibody from GeneTex.
SKU: GTX102321
CL0044416002A
Antibody from OriGene.
SKU: TA811161
CL0044416002A
Antibody from OriGene.
SKU: TA811180