Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

L0046099004-ABCF2-TA811105-02.jpg
CL0046099004A
Antibody from OriGene.
SKU: TA31156828 test change1
<a target="_blank" href="http://www.weather.com.cn/beijing/index.shtml">china</a>
CL0046099004A
Antibody from OriGene.
SKU: TA31234991 test change2
CL0039896418A
Antibody from OriGene.
SKU: TA22637061
CL0039896418A
Antibody from OriGene.
SKU: TA22402572
CL0039896418A
Antibody from OriGene.
SKU: TA22715224
CL0039896418A
Antibody from OriGene.
SKU: TA22480735
CL0039896418A
Antibody from OriGene.
SKU: TA22246246
CL0039896418A
Antibody from OriGene.
SKU: TA22558898
CL0039896418A
Antibody from OriGene.
SKU: TA22324409
CL0035743202A
Antibody from OriGene.
SKU: TA17165651
CL0015573702A
Antibody from GeneTex.
SKU: GTX101358
CL0035009011A
Antibody from OriGene.
SKU: TA15993206
CL0035009011A
Antibody from OriGene.
SKU: TA15758717kong test1
CL0035009011A
Antibody from OriGene.
SKU: TA15836880 test2
CL0035009011A
Antibody from OriGene.
SKU: TA15915043
CL0019478704A
Antibody from GeneTex.
SKU: GTX102321
CL0019478704A
Antibody from OriGene.
SKU: TA9193025
CL0019478704A
Antibody from GeneTex.
SKU: GTX102312
CL0044416002A
Antibody from OriGene.
SKU: TA28421123
CL0044416002A
Antibody from OriGene.
SKU: TA28499286