Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0044416002A
Antibody from OriGene.
SKU: TA811193
CL0031489312A
Antibody from OriGene.
SKU: TA500323
CL0031489312A
Antibody from OriGene.
SKU: TA500322
CL0033312302A
Antibody from OriGene.
SKU: TA503306
CL0033312302A
Antibody from OriGene.
SKU: TA503010
CL0033312302A
Antibody from OriGene.
SKU: TA503307
CL0033312302A
Antibody from OriGene.
SKU: TA503011
CL0033312302A
Antibody from OriGene.
SKU: TA503371
CL0033312302A
Antibody from OriGene.
SKU: TA502910
CL0033312302A
Antibody from OriGene.
SKU: TA503199
CL0033312302A
Antibody from OriGene.
SKU: TA502945
CL0042874604A
Antibody from OriGene.
SKU: TA500743
CL0042874604A
Antibody from OriGene.
SKU: TA500746
CL0014159205A
Antibody from GeneTex.
SKU: GTX113381
CL0013825202A
Antibody from OriGene.
SKU: TA504230
CL0013825202A
Antibody from OriGene.
SKU: TA504233
CL0013825202A
Antibody from OriGene.
SKU: TA504235
CL0034164501A
Antibody from OriGene.
SKU: TA503143
CL0034164501A
Antibody from OriGene.
SKU: TA503256
CL0034164501A
Antibody from OriGene.
SKU: TA503165