Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0044416002A
Antibody from OriGene.
SKU: TA28421123
CL0031489312A
Antibody from OriGene.
SKU: TA10599959
CL0031489312A
Antibody from OriGene.
SKU: TA10678122
CL0033312302A
Antibody from OriGene.
SKU: TA14117294
CL0033312302A
Antibody from OriGene.
SKU: TA13882805
CL0033312302A
Antibody from OriGene.
SKU: TA14195457
CL0033312302A
Antibody from OriGene.
SKU: TA13960968
CL0033312302A
Antibody from OriGene.
SKU: TA14273620
CL0033312302A
Antibody from OriGene.
SKU: TA14039131
CL0033312302A
Antibody from OriGene.
SKU: TA14351783
CL0033312302A
Antibody from OriGene.
SKU: TA13804642
CL0042874604A
Antibody from OriGene.
SKU: TA24981951
CL0042874604A
Antibody from OriGene.
SKU: TA25060114
CL0014159205A
Antibody from GeneTex.
SKU: GTX113381
CL0013825202A
Antibody from OriGene.
SKU: TA1611214
CL0013825202A
Antibody from OriGene.
SKU: TA1689377
CL0013825202A
Antibody from OriGene.
SKU: TA1533051
CL0034164501A
Antibody from OriGene.
SKU: TA14977087
CL0034164501A
Antibody from OriGene.
SKU: TA14742598
CL0034164501A
Antibody from OriGene.
SKU: TA15055250