Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0034164501A
Antibody from OriGene.
SKU: TA503165
CL0034164501A
Antibody from OriGene.
SKU: TA503269
CL0034164501A
Antibody from OriGene.
SKU: TA503196
CL0019924305A
Antibody from OriGene.
SKU: TA808476
CL0019924305A
Antibody from OriGene.
SKU: TA808471
CL0045437622A
Antibody from OriGene.
SKU: TA890099
CL0018809501A
Antibody from GeneTex.
SKU: GTX113329
CL0018809501A
Antibody from GeneTex.
SKU: GTX101070
CL0018809501A
Antibody from OriGene.
SKU: TA500967
CL0047992704A
Antibody from OriGene.
SKU: TA500950
CL0032354001A
Antibody from OriGene.
SKU: TA502048
CL0032354001A
Antibody from OriGene.
SKU: TA502123
CL0042375601A
Antibody from OriGene.
SKU: TA800336
CL0042375601A
Antibody from OriGene.
SKU: TA800333
CL0042375601A
Antibody from OriGene.
SKU: TA800334
CL0042375601A
Antibody from OriGene.
SKU: TA800330
CL0042375601A
Antibody from OriGene.
SKU: TA800335
CL0042375601A
Antibody from OriGene.
SKU: TA800331
CL0044455313A
Antibody from OriGene.
SKU: TA803662
CL0016435704A
Antibody from GeneTex.
SKU: GTX113385