Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0034164501A
Antibody from OriGene.
SKU: TA15133413
CL0034164501A
Antibody from OriGene.
SKU: TA14898924
CL0034164501A
Antibody from OriGene.
SKU: TA14977087
CL0019924305A
Antibody from OriGene.
SKU: TA9349351
CL0019924305A
Antibody from OriGene.
SKU: TA9427514
CL0045437622A
Antibody from OriGene.
SKU: TA29280916
CL0018809501A
Antibody from OriGene.
SKU: TA8489558
CL0018809501A
Antibody from GeneTex.
SKU: GTX101070
CL0018809501A
Antibody from GeneTex.
SKU: GTX113329
CL0047992704A
Antibody from OriGene.
SKU: TA33658044
CL0032354001A
Antibody from OriGene.
SKU: TA12866686
CL0032354001A
Antibody from OriGene.
SKU: TA12788523
CL0042375601A
Antibody from OriGene.
SKU: TA24122158
CL0042375601A
Antibody from OriGene.
SKU: TA24200321
CL0042375601A
Antibody from OriGene.
SKU: TA23965832
CL0042375601A
Antibody from OriGene.
SKU: TA24278484
CL0042375601A
Antibody from OriGene.
SKU: TA24043995
CL0042375601A
Antibody from OriGene.
SKU: TA24356647
CL0044455313A
Antibody from OriGene.
SKU: TA28655612
CL0016435704A
Antibody from GeneTex.
SKU: GTX113385