Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0045471713A
Antibody from OriGene.
SKU: TA501089
CL0045471713A
Antibody from OriGene.
SKU: TA501120
CL0037144601A
Antibody from OriGene.
SKU: TA502806
CL0017863902A
Antibody from OriGene.
SKU: TA804950
CL0017863902A
Antibody from GeneTex.
SKU: GTX109750
CL0017863902A
Antibody from OriGene.
SKU: TA804902
CL0017914608A
Antibody from OriGene.
SKU: TA809216
CL0017914608A
Antibody from OriGene.
SKU: TA809119
CL0017914608A
Antibody from OriGene.
SKU: TA809139
CL0017914608A
Antibody from OriGene.
SKU: TA809170
CL0015530804A
Antibody from GeneTex.
SKU: GTX129120
CL0017055101A
Antibody from GeneTex.
SKU: GTX119950
CL0017055101A
Antibody from GeneTex.
SKU: GTX132468
CL0048360204A
Antibody from OriGene.
SKU: TA503301
CL0048360204A
Antibody from OriGene.
SKU: TA503370
CL0048360204A
Antibody from OriGene.
SKU: TA503383
CL0048360204A
Antibody from OriGene.
SKU: TA503299
CL0013555804A
Antibody from GeneTex.
SKU: GTX130108
CL0012857507A
Antibody from OriGene.
SKU: TA810334
CL0015509901A
Antibody from OriGene.
SKU: TA890102