Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0031561713A
Antibody from OriGene.
SKU: TA11850567
CL0044689804A
Antibody from OriGene.
SKU: TA28890101
CL0023269109A
Antibody from OriGene.
SKU: TA10209144
CL0036650207A
Antibody from OriGene.
SKU: TA18572585
CL0036650207A
Antibody from OriGene.
SKU: TA18494422
CL0013399702A
Antibody from OriGene.
SKU: TA1142236
CL0016821301A
Antibody from OriGene.
SKU: TA5363038
CL0012690702A
Antibody from OriGene.
SKU: TA595095
CL0012690702A
Antibody from OriGene.
SKU: TA673258
CL0012690702A
Antibody from OriGene.
SKU: TA751421
CL0012690702A
Antibody from OriGene.
SKU: TA516932
CL0012690702A
Antibody from OriGene.
SKU: TA829584
CL0043378213A
Antibody from OriGene.
SKU: TA25998070
CL0017928101A
Antibody from GeneTex.
SKU: GTX113130
CL0017928101A
Antibody from GeneTex.
SKU: GTX100496
CL0017928101A
Antibody from GeneTex.
SKU: GTX111202
CL0019393801A
Antibody from GeneTex.
SKU: GTX131630