Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0018229501A
Antibody from OriGene.
SKU: TA806591
CL0018229501A
Antibody from OriGene.
SKU: TA806593
CL0018229501A
Antibody from OriGene.
SKU: TA806625
CL0018229501A
Antibody from OriGene.
SKU: TA806589
CL0016025908A
Antibody from GeneTex.
SKU: GTX105364
CL0044230411A
Antibody from OriGene.
SKU: TA501212
CL0044230411A
Antibody from OriGene.
SKU: TA501248
CL0044230411A
Antibody from OriGene.
SKU: TA501164
CL0044230411A
Antibody from OriGene.
SKU: TA501165
CL0047561715A
Antibody from OriGene.
SKU: TA504280
CL0047561715A
Antibody from OriGene.
SKU: TA504345
CL0012108012A
Antibody from GeneTex.
SKU: GTX110542
CL0019601102A
Antibody from Novus.
SKU: MAB707
CL0019601102A
Antibody from GeneTex.
SKU: GTX110543
CL0019601102A
Antibody from Novus.
SKU: AF-605-NA
CL0016886404A
Antibody from GeneTex.
SKU: GTX110723
CL0016886404A
Antibody from Novus.
SKU: AF705
CL0016886404A
Antibody from Novus.
SKU: NBP2-15722
CL0013017003A
Antibody from OriGene.
SKU: TA504351
CL0015117201A
Antibody from GeneTex.
SKU: GTX100205