Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0016645001A
Antibody from GeneTex.
SKU: GTX628777
CL0016645001A
Antibody from GeneTex.
SKU: GTX113400
CL0013571301A
Antibody from GeneTex.
SKU: GTX117561
CL0016844004A
Antibody from GeneTex.
SKU: GTX110541
CL0016844004A
Antibody from GeneTex.
SKU: GTX108624
CL0017814201A
Antibody from GeneTex.
SKU: GTX131669
CL0017814201A
Antibody from GeneTex.
SKU: GTX628472
CL0017814201A
Antibody from OriGene.
SKU: TA6457320
CL0017814201A
Antibody from GeneTex.
SKU: GTX102111
CL0017814201A
Antibody from GeneTex.
SKU: GTX628895
CL0017814201A
Antibody from GeneTex.
SKU: GTX112893
CL0015450101A
Antibody from OriGene.
SKU: TA3096311
CL0015450101A
Antibody from OriGene.
SKU: TA3174474
CL0046422315A
Antibody from OriGene.
SKU: TA31625806
CL0046422315A
Antibody from OriGene.
SKU: TA31703969
CL0047907512A
Antibody from OriGene.
SKU: TA33423555
CL0047907512A
Antibody from OriGene.
SKU: TA33189066
CL0047907512A
Antibody from OriGene.
SKU: TA33501718
CL0047907512A
Antibody from OriGene.
SKU: TA33267229
CL0047907512A
Antibody from OriGene.
SKU: TA33345392