Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0047907512A
Antibody from OriGene.
SKU: TA33267229
CL0041466616A
Antibody from OriGene.
SKU: TA23184202
CL0013259401A
Antibody from GeneTex.
SKU: GTX629627
CL0013259401A
Antibody from GeneTex.
SKU: GTX112898
CL0013259401A
Antibody from GeneTex.
SKU: GTX629543
CL0018196903A
Antibody from GeneTex.
SKU: GTX129902
CL0018196903A
Antibody from GeneTex.
SKU: GTX129903
CL0019254901A
Antibody from OriGene.
SKU: TA9036699
CL0021596405A
Antibody from OriGene.
SKU: TA9974655
CL0021596405A
Antibody from OriGene.
SKU: TA10052818
CL0021596405A
Antibody from OriGene.
SKU: TA9896492
CL0042723101A
Antibody from OriGene.
SKU: TA24825625
CL0045745822A
Antibody from OriGene.
SKU: TA30609687
CL0045745822A
Antibody from OriGene.
SKU: TA30453361
CL0045745822A
Antibody from OriGene.
SKU: TA30531524
CL0012675802A
Antibody from GeneTex.
SKU: GTX132204
CL0012675802A
Antibody from OriGene.
SKU: TA360606
CL0012675802A
Antibody from GeneTex.
SKU: GTX70100
CL0012675802A
Antibody from GeneTex.
SKU: GTX113055
CL0012675802A
Antibody from OriGene.
SKU: TA282443