Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0042427306A
Antibody from OriGene.
SKU: TA24512973
CL0042427306A
Antibody from OriGene.
SKU: TA24591136
CL0042427306A
Antibody from OriGene.
SKU: TA24669299
CL0042427306A
Antibody from OriGene.
SKU: TA24434810
CL0042427306A
Antibody from OriGene.
SKU: TA24747462
CL0017333301A
Antibody from OriGene.
SKU: TA5910179
CL0016644806A
Antibody from OriGene.
SKU: TA4972223
CL0016644806A
Antibody from GeneTex.
SKU: GTX103954
CL0023189705A
Antibody from OriGene.
SKU: TA10130981
CL0015793106A
Antibody from OriGene.
SKU: TA3799778
CL0015793106A
Antibody from OriGene.
SKU: TA3643452
CL0015793106A
Antibody from OriGene.
SKU: TA3721615
CL0017574902A
Antibody from OriGene.
SKU: TA6300994
CL0017574902A
Antibody from OriGene.
SKU: TA6379157
CL0017574902A
Antibody from GeneTex.
SKU: GTX633010
CL0017574902A
Antibody from OriGene.
SKU: TA6222831
CL0012235710A
Antibody from OriGene.
SKU: TA264698
CL0012235710A
Antibody from OriGene.
SKU: TA186535
CL0012235710A
Antibody from OriGene.
SKU: TA108372
CL0018583004A
Antibody from GeneTex.
SKU: GTX113680