Knockout validation as standard antibody validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein. In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

See all the knockout validation data below

CL0018583004A
Antibody from GeneTex.
SKU: GTX113680
CL0018583004A
Antibody from GeneTex.
SKU: GTX113681
CL0037222601A
Antibody from OriGene.
SKU: TA500062
CL0037222601A
Antibody from OriGene.
SKU: TA500063
CL0035224113A
Antibody from OriGene.
SKU: TA503387
CL0035224113A
Antibody from OriGene.
SKU: TA503421
CL0035224113A
Antibody from OriGene.
SKU: TA503388
CL0035224113A
Antibody from OriGene.
SKU: TA503422
CL0035224113A
Antibody from OriGene.
SKU: TA503390
CL0035224113A
Antibody from OriGene.
SKU: TA503423
CL0035224113A
Antibody from OriGene.
SKU: TA503391
CL0044365103A
Antibody from Novus.
SKU: MAB4145
CL0044365103A
Antibody from OriGene.
SKU: TA502194
CL0044365103A
Antibody from OriGene.
SKU: TA502195
CL0044365103A
Antibody from OriGene.
SKU: TA502244
CL0044365103A
Antibody from OriGene.
SKU: TA502348
CL0033179101A
Antibody from OriGene.
SKU: TA812139
CL0013664006A
Antibody from GeneTex.
SKU: GTX129127
CL0011456805A
Antibody from GeneTex.
SKU: GTX133182
Antibody from GeneTex
CL0011456805A
Antibody from GeneTex.
SKU: GTX109162