CRISPR/Cas9是新一代的基因组编辑技术,应用该技术可以在任意细胞中进行基因敲除、基因敲入、定点突变等操作。由于其简捷性和可扩展性,被广泛应用于各种生物研究中。

基因修饰的细胞系是研究基因功能、构建疾病模型、研究生物医药、发现药物靶点等研究中非常重要的工具。博雅辑因不仅可以提供超过1200种基因敲除细胞系现货,同时也提供基因编辑细胞系定制服务,包括设计sgRNA,构建质粒等一系列相关服务。

参考下列表格中的基因编辑服务项目,针对每个项目我们将由专业的技术人员对接,提供详细专业的评估和解答。下载EdiGene Inquiry 表格,填写您的需求,并将需求发送到邮箱bd@edigene.com中,我们的人员会尽快联系您!

 

CRISPR 细胞系定制服务 周期 起始价格^ 详情
基因敲除细胞系
· Single-gene knockout
· Multi-gene knockout
· Large segment deletes
10-18 周
(Product delivery time depends on the status of cell growth, the ability of single cloning, the level of the target knockout and the transfection, and so on.)

$3,000 简单的基因敲除细胞系

· Client-specified cell lines *and gene targets;

· A variety of transfection methods;

· Multiple reporter marks to choose from;

· Bi-weekly progress report;

· Delivery of monoclonal gene Knockout cell;

· PCR and sequencing verification results report;

· sgRNA sequence design report.

$5,000 较难的基因敲除细胞系

基因敲入细胞系
· Point mutation
16-25周
(Project delivery time depends on the status of cell growth, the ability of single cloning, and so on.)
询价 Client-specified cell lines *and gene targets; Efficient sgRNA and homologous arm design; A variety of transfection methods; Multiple reporter marks to choose from; Bi-weekly progress report; Delivery of monoclonal gene Knockout cell; PCR and sequencing verification results report; sgRNA sequence design report; Professional technical platform guarantee, Peking university technical background.

报告细胞系
· Resistance marker
· Fluorescent marker
· Toxin marker
· Other report marker

根据项目实际情况 询价 Stable reporting system cell line; Sequencing verification report; Assisted high-throughput library screening; High efficiency infection

*It is preferred that customers provide their own cell lines, but cell lines can also be purchased by EdiGene for an additional fee.

^Prices may vary based on project specifications.

附加服务 · 额外的单克隆基因编辑细胞系
· 细胞遗传信息确认报告
· STR检测报告

工作流程

workflow

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或下载 “EdiGene Inquiry” 表格,填写您的需求,发送到 bd@edigene.com