CRISPR/Cas9鏄柊涓浠g殑鍩哄洜缁勭紪杈戞妧鏈紝搴旂敤璇ユ妧鏈彲浠ュ湪浠绘剰缁嗚優涓繘琛屽熀鍥犳暡闄ゃ佸熀鍥犳暡鍏ャ佸畾鐐圭獊鍙樼瓑鎿嶄綔銆傜敱浜庡叾绠鎹锋у拰鍙墿灞曟э紝琚箍娉涘簲鐢ㄤ簬鍚勭鐢熺墿鐮旂┒涓

鍩哄洜淇グ鐨勭粏鑳炵郴鏄爺绌跺熀鍥犲姛鑳姐佹瀯寤虹柧鐥呮ā鍨嬨佺爺绌剁敓鐗╁尰鑽佸彂鐜拌嵂鐗╅澏鐐圭瓑鐮旂┒涓潪甯搁噸瑕佺殑宸ュ叿銆傚崥闆呰緫鍥犱笉浠呭彲浠ユ彁渚涜秴杩2000绉嶅熀鍥犳暡闄ょ粏鑳炵郴鐜拌揣锛屽悓鏃朵篃鎻愪緵鍩哄洜缂栬緫缁嗚優绯诲畾鍒舵湇鍔★紝鍖呮嫭璁捐sgRNA锛屾瀯寤鸿川绮掔瓑涓绯诲垪鐩稿叧鏈嶅姟銆

鍙傝冧笅鍒楄〃鏍间腑鐨勫熀鍥犵紪杈戞湇鍔¢」鐩紝閽堝姣忎釜椤圭洰鎴戜滑灏嗙敱涓撲笟鐨勬妧鏈汉鍛樺鎺ワ紝鎻愪緵璇︾粏涓撲笟鐨勮瘎浼板拰瑙g瓟銆備笅杞EdiGene Inquiry 琛ㄦ牸锛屽~鍐欐偍鐨勯渶姹傦紝骞跺皢闇姹傚彂閫佸埌閭bd@edigene.com涓紝鎴戜滑鐨勪汉鍛樹細灏藉揩鑱旂郴鎮紒

CRISPR 缁嗚優绯诲畾鍒舵湇鍔 鍛ㄦ湡 璧峰浠锋牸^ 璇︽儏
鍩哄洜鏁查櫎缁嗚優绯
路 Single-gene knockout
路 Multi-gene knockout
路 Large segment deletes
10-18 鍛
(Product delivery time depends on the status of cell growth, the ability of single cloning, the level of the target knockout and the transfection, and so on.)

璇环

绠鍗曠殑鍩哄洜鏁查櫎缁嗚優绯

路 Client-specified cell lines *and gene targets;

路 A variety of transfection methods;

路 Multiple reporter marks to choose from;

路 Bi-weekly progress report;

路 Delivery of monoclonal gene Knockout cell;

路 PCR and sequencing verification results report;

路 sgRNA sequence design report.

璇环

杈冮毦鐨勫熀鍥犳暡闄ょ粏鑳炵郴

鍩哄洜鏁插叆缁嗚優绯
路 Point mutation
16-25鍛
锛圥roject delivery time depends on the status of cell growth, the ability of single cloning, and so on.锛
璇环 Client-specified cell lines *and gene targets; Efficient sgRNA and homologous arm design; A variety of transfection methods; Multiple reporter marks to choose from; Bi-weekly progress report; Delivery of monoclonal gene Knockout cell; PCR and sequencing verification results report; sgRNA sequence design report; Professional technical platform guarantee, Peking university technical background.

鎶ュ憡缁嗚優绯
路 Resistance marker
路 Fluorescent marker
路 Toxin marker
路 Other report marker

鏍规嵁椤圭洰瀹為檯鎯呭喌 璇环 Stable reporting system cell line; Sequencing verification report; Assisted high-throughput library screening; High efficiency infection

*It is preferred that customers provide their own cell lines, but cell lines can also be purchased by EdiGene for an additional fee.

^Prices may vary based on project specifications.

闄勫姞鏈嶅姟 路 棰濆鐨勫崟鍏嬮殕鍩哄洜缂栬緫缁嗚優绯
路 缁嗚優閬椾紶淇℃伅纭鎶ュ憡
路 STR妫娴嬫姤鍛

宸ヤ綔娴佺▼

workflow

璁㈣喘

鐢佃瘽锛010-8073 3899 鍒嗘満鍙 802

鎴栦笅杞 鈥EdiGene Inquiry鈥 琛ㄦ牸锛屽~鍐欐偍鐨勯渶姹傦紝鍙戦佸埌聽bd@edigene.com