CRISPR-generated Knockout Cell Lines (KO Cell Lines): EdiGene continuously optimizes our CRISPR/Cas9-based genome editing technology to generate stable, edited mammalian cell lines. EdiGene’s validated knockout cell lines may be used in any number of applications where gene knockout is desired: biomarker discovery, drug target screening, biological pathway analysis, gene function analysis, and antibody validation. Learn for yourself why “EdiGene KO Validated” is rapidly becoming the industry gold standard for validating high quality polyclonal and monoclonal antibodies. You may choose from one or more of over 1500 (and rapidly growing!) ready-made knockout cell lines. Unlike other ready-made CRISPR-generated knockout cell lines, EdiGene’s knockout cell lines are widely used cell lines (HeLa and 293T) that are more likely to be applicable to your studies.
CRISPR Genome Editing Tools: EdiGene provides you with the flexibility to perform the CRISPR/Cas9 genome editing that suits your applications. With the Cas9 Expressing Stable Cell Lines you can create knockout or knock-in stable cells using your own sgRNA and homologous arm designs. You also may purchase the validated sgRNA we used to generate the knockout cell lines so that you may perform stable knockouts in your cell line of choice.
CRISPR Genome Editing Services: Can’t find the ready-made cell line that fits your needs? EdiGene will design and generate the desired genome editing in your cell line of choice: knockout or knock-in. EdiGene will design efficient sgRNA and donor DNA, determine and utilize the best transfection method for your cell type, screen and perform single-cell clonal selection. The edited genome will be thoroughly validated via PCR analysis and Sanger sequencing. Because generating and validating genome-edited cell lines can take weeks to several months, EdiGene is committed to being transparent through the entire process and will provide regular progress reports (every 2 -3 weeks) so you may monitor and stay abreast of the process.
High Throughput Genetic Screening: EdiGene integrates CRISPR/Cas9 genome editing and high throughput loss of function genetic screening to accelerate new drug target discovery and further clarify mechanisms of action. Performing loss-of-function screens with CRISPR-generated libraries has many advantages over using RNAi libraries including the ability to create stable and inheritable genome alterations, complete knockout instead of partially inhibition of gene function, and clear-cut results. Creating stable and inheritable gene knockouts allows analysis of key phenotypes that may take a longer period of time to manifest. You may choose from any of our ready-made sgRNA libraries or have us create a custom library to your specifications. Additionally, with extensive experience in both CRISPR/Cas9 genome editing and high-throughput screening, EdiGene can perform the screening protocol for you.